fcs module Search Results


the fcs module  (Carl Zeiss)
90
Carl Zeiss the fcs module
The Fcs Module, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/the fcs module/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
the fcs module - by Bioz Stars, 2026-06
90/100 stars
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fcs module  (PicoQuant inc)
90
PicoQuant inc fcs module
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
Fcs Module, supplied by PicoQuant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fcs module/product/PicoQuant inc
Average 90 stars, based on 1 article reviews
fcs module - by Bioz Stars, 2026-06
90/100 stars
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alba fcs correlation module  (ISS Inc)
90
ISS Inc alba fcs correlation module
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
Alba Fcs Correlation Module, supplied by ISS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alba fcs correlation module/product/ISS Inc
Average 90 stars, based on 1 article reviews
alba fcs correlation module - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
fcs/flim module  (PicoQuant inc)
90
PicoQuant inc fcs/flim module
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
Fcs/Flim Module, supplied by PicoQuant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fcs/flim module/product/PicoQuant inc
Average 90 stars, based on 1 article reviews
fcs/flim module - by Bioz Stars, 2026-06
90/100 stars
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two-channel fcs module (dichroic mirror and band pass emission filters 505–580, 605–660 nm)  (ISS Inc)
90
ISS Inc two-channel fcs module (dichroic mirror and band pass emission filters 505–580, 605–660 nm)
Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . <t>Fluorescent</t> <t>microscope</t> images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for <t>FCS</t> studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.
Two Channel Fcs Module (Dichroic Mirror And Band Pass Emission Filters 505–580, 605–660 Nm), supplied by ISS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/two-channel fcs module (dichroic mirror and band pass emission filters 505–580, 605–660 nm)/product/ISS Inc
Average 90 stars, based on 1 article reviews
two-channel fcs module (dichroic mirror and band pass emission filters 505–580, 605–660 nm) - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
enhanced software module fcs for zen system  (Carl Zeiss)
N/A
Enhanced Software module FCS for ZEN system The module enables interactive and global fitting and contains extended and self-defined fit models.
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zen module fcs hardware license key  (Carl Zeiss)
N/A
ZEN Module FCS Hardware License Key Fluorescence Correlation Spectroscopy for GaAsP detectors and APD, single molecule analyses FCS, spectral FCS and FCCS
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Image Search Results


Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . Fluorescent microscope images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for FCS studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.

Journal: FEBS Open Bio

Article Title: Optimizing fluorescent protein expression for quantitative fluorescence microscopy and spectroscopy using herpes simplex thymidine kinase promoter sequences

doi: 10.1002/2211-5463.12432

Figure Lengend Snippet: Confocal laser scanning microscopy images of mC herry expressed using a variety of promoter sequences. Rows, panels 1 to 4 (top to bottom), display the expression of mC herry in the four cell lines, human HeLa CCL 2, human U2 OS , mouse NIH 3T3 cells and mouse stem cells BALB /c, as indicated after the transfection with the indicated expression vectors. Columns present promoter sequences controlling expression of mC herry as shown, which are in detail described in Fig. . Fluorescent microscope images show comparison of mC herry fluorescence expressed by different promoters and deletion mutant constructs described in this study. Most of the cells show very high expression of mC herry protein when transfected with plasmids containing CMV and TK Δ SS promoters. The full‐length TK promoter shows a reduced but still high expression level, whereas constructs TK ST and TKTSC show optimal fluorescence for FCS studies. Images shown are representative of multiple experiments (at least three independent transfections). The lower right image contains the scale bar = 20 μm valid for all other images. The images present cells with representative mC herry expression levels for each promoter construct and cell line. To make the intensities of mC herry comparable, the images were taken with the same parameters including amplification settings of the detectors and the later handling of the images for all promoter constructs and cell lines. Therefore, the intensities of red fluorescence for promoter constructs CMV and TK Δ SS are very high meaning high mC herry expression levels. In contrast, the fluorescent signals seemed to be barely detectable for TK ST and TKTSC promoter constructs, but both these mC herry levels are optimal for FCS as discussed in the main text.

Article Snippet: The live‐cell confocal imaging and FCS measurements were carried out using FV1000 inverted epifluorescence microscope (Olympus, Hamburg) equipped with FCS module (PicoQuant GmbH, Berlin) and an UplanSApo 60 × water immersion objective lens (NA 1.2).

Techniques: Confocal Laser Scanning Microscopy, Expressing, Transfection, Microscopy, Fluorescence, Mutagenesis, Construct, Amplification